Tilapia lake virus (TiLV) is an emerging virus that causes syncytial hepatitis of tilapia with mortalities of up to 90%. Recent disease outbreaks in Thai tilapia farms have been associated with high cumulative mortalities and histopathological features typical of SHT. Infection has now been confirmed. The semi-nested RT-PCR protocol described here may be used freely for non-commercial applications to detect TiLV. The authors urge laboratories in Asia to test for TiLV when abnormal tilapia mortality occurs.
The development of resistant strains of disease-causing microorganisms is an important health issue of global concern. When microbes such as bacteria, fungi, parasites, and viruses become resistant to antimicrobial substances, the diseases they may cause become more difficult or impossible to treat. Resistance is developed by the indiscriminate use of antimicrobials and places human health at risk.
The discovery of antibiotics revolutionized medicine, creating a belief that a 'magic bullet' had finally been found to control bacterial diseases. Antibiotics, a class of antimicrobial agents, kill or inhibit the growth of bacteria, but they have no significant effect on other types of microorganisms such as viruses.
We invite you to join us for the 10th Symposium on Diseases in Asian Aquaculture (DAA10), the popular triennial event of the Fish Health Section of the Asian Fisheries Society (FHS-AFS), to be held from 28 August to 1 September 2017 at The Anvaya Beach Resort, Kuta, Bali, Indonesia.
With the chosen theme of “Enhancing Aquatic Animal Health Research and Services through Public-Private Sector Partnerships” we anticipate to cover topics from classic parasitic, bacterial and viral diseases to emerging trends and cutting-edge research in aquatic animal health.
Three satellite workshops: FAO’s Inter-regional Workshop, NACA’s Meeting of the Asia Regional Advisory Group on Aquatic Animal Health, and a Bioinformatics Workshop are being organised back-to-back with DAA10.
Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is a newly emerging disease of cultivated shrimp in Asia. Current evidence indicates that it can be associated with severe growth retardation that may not be clearly evident until the second month of culture and that it may even cause low continuous mortality in the case of very severe infections.
Here we present a new method for detecting Enterocytozoon hepatopenaei (EHP) that has superior specificity to the first generation SSU-PCR developed in 2009 when the genetic information of EHP was still limited. Due to the urgency in stemming losses to HPM, we have decided to release this method for free, non-commercial use to the global shrimp farming community.
The second generation EHP detection method presented here is based on a gene encoding a spore wall protein (SWP) of EHP (SWP-PCR). Results from our laboratory work revealed, in contrast to SSU-PCR, that the SWP-PCR method did not give cross reactions with DNA from crabs infected with H. eriocheir and E. canceri. From these results, we recommend that the new SWP-PCR method replace the first generation SSU-PCR method.
The sequences of the primers for the SWP-PCR method (nested PCR) are given below and can be used freely for non-commercial applications to detecting EHP. Please contact Centex Shrimp (ornchuma.its 'at' mahidol.ac.th) to obtain a free positive control plasmid (pGEM-EHPSWP).
Prizes include: Power banks, fitness watches, USB sticks and t-shirts
FishVet Group Asia Limited is collecting data on the geographic spread and impact of EHP (Enterocytozoon hepatopenaei).
All farmers who complete a short survey form by 30 June 2016 will be included in a prize draw with the following prizes:
All data will be kept strictly confidential.