Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is a newly emerging disease of cultivated shrimp in Asia. Current evidence indicates that it can be associated with severe growth retardation that may not be clearly evident until the second month of culture and that it may even cause low continuous mortality in the case of very severe infections.
Here we present a new method for detecting Enterocytozoon hepatopenaei (EHP) that has superior specificity to the first generation SSU-PCR developed in 2009 when the genetic information of EHP was still limited. Due to the urgency in stemming losses to HPM, we have decided to release this method for free, non-commercial use to the global shrimp farming community.
The second generation EHP detection method presented here is based on a gene encoding a spore wall protein (SWP) of EHP (SWP-PCR). Results from our laboratory work revealed, in contrast to SSU-PCR, that the SWP-PCR method did not give cross reactions with DNA from crabs infected with H. eriocheir and E. canceri. From these results, we recommend that the new SWP-PCR method replace the first generation SSU-PCR method.
The sequences of the primers for the SWP-PCR method (nested PCR) are given below and can be used freely for non-commercial applications to detecting EHP. Please contact Centex Shrimp (ornchuma.its 'at' mahidol.ac.th) to obtain a free positive control plasmid (pGEM-EHPSWP).
Prizes include: Power banks, fitness watches, USB sticks and t-shirts
FishVet Group Asia Limited is collecting data on the geographic spread and impact of EHP (Enterocytozoon hepatopenaei).
All farmers who complete a short survey form by 30 June 2016 will be included in a prize draw with the following prizes:
All data will be kept strictly confidential.
The Second International Technical Seminar/Workshop on AHPND: There is a way Forward! will be held from 23-25 June 2016 at the Sukosol Hotel in Bangkok, Thailand.
The purpose of the meeting is to update knowledge and exchange experiences in dealing with AHPND, to validate current concepts and models under different systems and environmental conditions and to put into action the responsibilities of the different sectors (i.e. government, producer and academe) as a way forward to deal with AHPND.
The meeting will consist of three technical sessions looking at EMS/AHPND through the lens of the industry, the academe and the government sectors and a fourth session on the way forward.
All interested stakeholders are welcome to attend. A catering fee of US$40 / day applies (includes lunch plus morning and afternoon tea). Reservations will be accepted until close of business on 10 June; places are limited.
Due to the world’s rapidly growing population, which is expected to peak somewhere around 9.5 billion, food production will need to be massively increased over the next few decades. This increase must be achieved without further degrading the environment. The unit environmental footprint of food production must be significantly reduced from where it is today. This concept, termed sustainable intensification, applies as much to aquaculture as it does to other agricultural sectors.
A new method for the detection of AHPND-bacteria (AP4) has been published and is available for download.
The advantage of the AP4 method over the previously published AP3 method is that the it has 100 times higher sensitivity. Because of its higher sensitivity, the bacterial culture enrichment step needed when using the AP3 with low levels of AHPND bacteria may be omitted. However, the AP4 method should not be considered as a replacement for the AP3 method but simply as an alternative choice for the users to choose should they need a more sensitive detection method.