Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is a newly emerging disease of cultivated shrimp in Asia. Current evidence indicates that it can be associated with severe growth retardation that may not be clearly evident until the second month of culture and that it may even cause low continuous mortality in the case of very severe infections.
Here we present a new method for detecting Enterocytozoon hepatopenaei (EHP) that has superior specificity to the first generation SSU-PCR developed in 2009 when the genetic information of EHP was still limited. Due to the urgency in stemming losses to HPM, we have decided to release this method for free, non-commercial use to the global shrimp farming community.
The second generation EHP detection method presented here is based on a gene encoding a spore wall protein (SWP) of EHP (SWP-PCR). Results from our laboratory work revealed, in contrast to SSU-PCR, that the SWP-PCR method did not give cross reactions with DNA from crabs infected with H. eriocheir and E. canceri. From these results, we recommend that the new SWP-PCR method replace the first generation SSU-PCR method.
The sequences of the primers for the SWP-PCR method (nested PCR) are given below and can be used freely for non-commercial applications to detecting EHP. Please contact Centex Shrimp (ornchuma.its 'at' mahidol.ac.th) to obtain a free positive control plasmid (pGEM-EHPSWP).
Prizes include: Power banks, fitness watches, USB sticks and t-shirts
FishVet Group Asia Limited is collecting data on the geographic spread and impact of EHP (Enterocytozoon hepatopenaei).
All farmers who complete a short survey form by 30 June 2016 will be included in a prize draw with the following prizes:
All data will be kept strictly confidential.
The Second International Technical Seminar/Workshop on AHPND: There is a way Forward! will be held from 23-25 June 2016 at the Sukosol Hotel in Bangkok, Thailand.
The purpose of the meeting is to update knowledge and exchange experiences in dealing with AHPND, to validate current concepts and models under different systems and environmental conditions and to put into action the responsibilities of the different sectors (i.e. government, producer and academe) as a way forward to deal with AHPND.
The meeting will consist of three technical sessions looking at EMS/AHPND through the lens of the industry, the academe and the government sectors and a fourth session on the way forward.
All interested stakeholders are welcome to attend. A catering fee of US$40 / day applies (includes lunch plus morning and afternoon tea). Reservations will be accepted until close of business on 10 June; places are limited.
A global conference on climate change adaptation in fisheries and aquaculture will be held in Bangkok from 8-10 August. The "FishAdapt" conference is being organised by 21 partner organisations from around the world and sponsored by the governments of Japan, Norway, Thailand and the United States.
Climate change is altering aquatic ecosystems, driving shifts in physical and chemical processes, ecological communities and the distribution and abundance of species. These changes have implications for fisheries management, food security and the livelihoods of more than 600 million people world wide that are employed in fisheries and aquaculture or related industries.
This conference is targeted at scientists, development professionals and natural resource managers working in the context of fisheries, aquaculture, rural development and related fields. It will provide a forum to share practical experiences in understanding the vulnerabilities associated with climate change and the development of risk management and adaptation strategies.
The conference will bridge inter-disciplinary gaps and provide a wider, shared perspective on the issues and the current state of knowledge. If you would like to contribute to the development of the conference or to help shape the programme please contact Cassandra De Young (Cassandra.DeYoung 'at' fao.org) for more information.
The FishAdapt Conference website is now open for registration and submission of abstracts!
The International Institute of Knowledge Management of Sri Lanka invites you to submit original research papers for presentation to the 3rd International Conference on Fisheries and Aquaculture (ICFA 2016) to be held from 24 – 25 August 2016, in Negombo - Sri Lanka with the theme "Aquaculture as an imperative segment of blue economy and sustainable development goals". The deadline for submission of abstracts is 15 May 2016. For more information please visit the conference website for email abstract 'at' aquaconference.com.