Sustainable aquaculture and aquatic resources management

A new and improved PCR detection method for Enterocytozoon hepatopenaei (EHP) based on a gene encoding a spore wall protein

Author(s): Itsathitphaisarn, O, Jaroenlak, P., Sanguanrut, P., Salachan, P.V., Wiredu-Boakye, D., Williams, B.A.P, Stantiford, G.D., Flegel, T.W., Sritunyalucksana, K. | Published: 3/7/2016 | 3292 views

Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is a newly emerging disease of cultivated shrimp in Asia. Current evidence indicates that it can be associated with severe growth retardation that may not be clearly evident until the second month of culture and that it may even cause low continuous mortality in the case of very severe infections. 

Here we present a new method for detecting Enterocytozoon hepatopenaei (EHP) that has superior specificity to the first generation SSU-PCR developed in 2009 when the genetic information of EHP was still limited. Due to the urgency in stemming losses to HPM, we have decided to release this method for free, non-commercial use to the global shrimp farming community.   

The second generation EHP detection method presented here is based on a gene encoding a spore wall protein (SWP) of EHP (SWP-PCR). Results from our laboratory work revealed, in contrast to SSU-PCR, that the SWP-PCR method did not give cross reactions with DNA from crabs infected with H. eriocheir and E. canceriFrom these results, we recommend that the new SWP-PCR method replace the first generation SSU-PCR method.

The sequences of the primers for the SWP-PCR method (nested PCR) are given below and can be used freely for non-commercial applications to detecting EHP. Please contact Centex Shrimp (ornchuma.its 'at' mahidol.ac.th) to obtain a free positive control plasmid (pGEM-EHPSWP).

NACA Newsletter, January-June 2016

Published: 9/6/2016 | 1498 views
  • NACA conducts workshops on white spot disease and shrimp health management in I.R. Iran.
  • Don’t forget to register for the 11th Asian Fisheries and Aquaculture Forum!
  • NACA pays tribute to Professor H.P.C. Shetty – Patron of the Pillay Aquaculture Foundation.
  • EHP: Shrimp industry survey.
  • 3rd International Conference on Fisheries and Aquaculture, 24-25 August, Negombo, Sri Lanka.
  • Special Session on the Status of Aquatic Genetic Resources for Food and Agriculture.
  • Second International Technical Workshop on Acute Hepatopancreatic Necrosis Disease (AHPND).
  • Guidebook on Farmer-to-Farmer Extension Approach for Small-Scale Freshwater Aquaculture.
  • Sustainable intensification of aquaculture in the Asia-Pacific region.

Quarterly Aquatic Animal Disease Report Q3 2015

Published: 27/5/2016 | 1296 views

The regional Quarterly Aquatic Animal Disease (QAAD) reporting system has been implemented since the second quarter of 1998 and continues to provide a useful mechanism for aquatic animal disease information sharing amongst 21 participating governments in the Asia-Pacific region. The QAAD reporting system is a joint activity between NACA, FAO and OIE Regional Representation (Tokyo). The 2015/3 QAAD report, 69th in the series, includes disease information from 9 governments.

This issue's foreword discusses the highlights of the 14th Meeting of the Asian REgional Advisory Group on Aquatic Animal Health and changes to the Asia-Pacific QAAD report.

Quarterly Aquatic Animal Disease Report Q4 2015

Published: 27/5/2016 | 1128 views

The regional Quarterly Aquatic Animal Disease (QAAD) reporting system has been implemented since the second quarter of 1998 and continues to provide a useful mechanism for aquatic animal disease information sharing amongst 21 participating governments in the Asia-Pacific region. The QAAD reporting system is a joint activity between NACA, FAO and OIE Regional Representation (Tokyo). The 2015/4 QAAD report, 70th in the series, includes disease information from 9 governments.

This issue's foreword discusses a new viral disease of farmed tilapia: Tilapia lake virus.

Forteenth Meeting of the Asia Regional Advisory Group on Aquatic Animal Health

Published: 28/3/2016 | 1462 views

This report was prepared by the 14th Asia Regional Advisory Group on Aquatic Animal Health (AG) that met at KU Home, Kasetsart University Campus on 23rd to 25th November, 2015. The report provides:

  • A progress report on NACA’s Regional Aquatic Animal Health Programme.
  • A discussion of OIE standards and global issues in aquatic animal health.
  • A review of regional aquatic animal disease status, including of shrimp, finfish, amphibian and molluscan diseases.
  • Reports on the aquatic animal health programmes of partner agencies.
  • Revisions to the regional aquatic animal disease reporting system, including the listing of HPM-EHP.

Guidelines for hatchery production of Pa Phia fingerlings in Lao PDR

Author(s): Ingram, B.A., Chanthavong, K., Nanthalath, T., De Silva, S.S. | Published: 4/2/2016 | 1532 views

This manual provides basic guidelines for the hatchery production of Pa Phia (Labeo chrysophekadion) fingerlings. It provides information on managing and spawning broodstock, genetic guidelines, egg incubation, hatching larviculture and fry rearing. The manual draws on published information on Pa Phia; results of artificial propagation trials conducted on Pa Phia during two projects funded by the Australian Centre for International Agricultural Research and the experiences of technicians at two government hatcheries.

Final Draft Code of Pratice for Trans-boundary Movement of Aquatic Organisms in the Lower Mekong Delta

Published: 28/1/2016 | 1467 views

This Code of Practice is prepared to promote or ensure compliance to World Trade Organisation-Sanitary and Phytosanitary (WTO-SPS) measures for the movement of live aquatic organisms in the Lower Mekong Basin (LMB). The long-term goals of the Code are to achieve environmental protection and management, biodiversity conservation as well as prevention of spread of disease epizootics. Most of the important points listed in this Code are based on the inputs of MRC Member Countries through national reviews on the Impacts of Exotic Species on Natural Environment and Aquaculture.

Aquaculture Asia Magazine, Vol. XX No. 1, January-March 2015

Published: 24/12/2015 | 2433 views

Sustainable aquaculture

Peter Edwards writes on rural aquaculture: Further training provided to aquaculturists in Fiji.

Spatial planning for sustainable coastal shrimp production.
Olivier M. Joffre, Pham Dang Tri, Tran Thi Phung Ha, Roel H. Bosma

Research and farming techniques

Availability of grouper (Serranidae) fingerlings and seed in the coral reef of Son Tra Peninsula, central Viet Nam.
Nguyen Thi Tuong Vi, Vo Van Quang, Le Thi Thu Thao, Tran Thi Hong Hoa, Tran Cong Thinh

People in aquaculture
Small-scale carp seed production through portable FRP hatchery at Khanguri, Odisha: A case of technology transfer in remote and inaccessible village.
B. C. Mohapatra, N. K. Barik, S. K. Mahanta, H. Sahu, B. Mishra and D. Majhi

NACA Newsletter

  • Regional consultation on culture-based fisheries developments in Asia.
  • Gender Assessment Synthesis Workshop.
  • NACA participation in the 5th Global Symposium on Gender in Aquaculture and Fisheries, Lucknow, India.
  • Broodstock management in aquaculture: Long term effort required for regional capacity building.
  • Urgent appeal to control spread of the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP).

Quarterly Aquatic Animal Disease Report Q2 2015

Published: 7/12/2015 | 1608 views

The regional Quarterly Aquatic Animal Disease (QAAD) reporting system has been implemented since the second quarter of 1998 and continues to provide a useful mechanism for aquatic animal disease information sharing amongst 21 participating governments in the Asia-Pacific region. The QAAD reporting system is a joint activity between NACA, FAO and OIE Regional Representation (Tokyo). The 2015/2 QAAD report, 68th in the series, includes disease information from 13 governments. This issue's foreword discusses harmonisation of aquaculture certification in the ASEAN.

Hepatopancreatic microsporidiosis caused by Enterocytozoon hepatopenaei (EHP)

Published: 25/10/2015 | 2631 views

This disease card describes the diagnosis and range of a microsporidian shrimp pathogen, Enterocytozoon hepatopenaei (EHP), first discovered in Penaeus monodon in Thailand in 2004. It infects only the tubule epithelial cells of the hepatopancreatic tissue of shrimp.

EHP was later found to also infect P. vannamei cultivated in Thailand and is suspected to have been reported from P. japonicus in Australia in 2001. EHP has been reported from Vietnam and is associated with white faeces syndrome. It resembles an unnamed microsporidian reported in the hepatopancreas of P. monodon in Malaysia in 1989 and in P. japonicus in Australia in 2001. PCR positive results have also been obtained from P. vannamei cultivated in Indonesia and India, thus it is probable that EHP is endemic in the Australasian region.

 

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